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#11
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http://www.reefcentral.com/forums/sh...r&pagenumber=1
No mushrooms regrew after 1 month. Any success stories out there? |
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#12
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I'm sure the blender thing would work just fine with a quick jog on low for a whack of small tissue, however if your puree the shrooms to the point that there is no living tissue left and simply just sludge then I could see it not working
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cheers, Rich all that we do is touched with ocean, yet we remain on the shore of what we know http://www.advancedaquarist.com/2005/5/aquarium |
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#13
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You're welcome!
I'm not really sure about having small chunks of tissue or not. Theoretically you only need one cell, so as long as you have some microscopic cells floating around in the solution you technically could have a mushroom grow out of that cell. When you liquify the musroom you most likely are disrupting a lot of the cells causing their cytoplasm to leak out which would kill the cell (you'd be disrupting the cell's membrane). But if any cells survived (you wouldnt be able to see them cuz they're microscopic) you could get mushrooms. But if you did leave small chunks in the solution you would for sure know that some of the cells weren't killed, and would have an even greater chance of musroom regrowth. You also have to think about denaturation of the proteins in the mushroom. Heat isn't the only thing that denatures proteins causing inactivation. If you stir proteins too much (aka the really high stirring and blending in the blender) it can also cause the proteins to turn inside out and misfold and denature. This would cause inactivation and the cells wouldn't survive and create baby mushrooms. I'm really interested to see if it works now. In all theory one cell should cause one mushroom regardless of large chunks in solution or not. People haven't had success trying it, but i wonder if the feat can be done. Cheers.
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Krissy - student, ecology/biology major, loves nature!
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#14
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Quote:
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#15
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Quote:
I think a better way would be to chop with a sharp chef's knife instead of a blender. I agree that mincing would kill too much cellular tissue. In my new tank I'm going to build a little bowl in my tank out of small peices of live rock, that way the shrooms have something to grasp without being blown all over the tank. I'll call it mushroom soup!
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THE BARQUARIUM: 55 gallon cube - 50 lbs LR - ASM G3 skimmer - 30 Gallon sump - 22 Gallon refugium / frag tank - 4x 24 watt HO T5's - Mag 9.5 return - Pin Point PH monitor - 400 watt XM 20K MH in Lumenarc reflector - Dual stage GFO/NO3 media reactor - 6 stage RODI auto top up -Wavemaster Pro running 3 Koralia 2's. Fully stocked with fish, corals and usually some fine scotch http://www.canreef.com/vbulletin/showthread.php?t=55041 |