Live Phytoplankton saltwater culture / HELP

[monocus]

take the nanocloropus,add the same amount of water,and add a couple of drops of micro algae grow.start your experiment with your yeast culture co2 and don't add anymore micro algae grow.in another container do the same as above,but add a few drops of micro algae grow daily-you now have two cultures-1 being fed and the other relying on just co2.you can make a density stick for counting your cultures by using a small plastic ruler with a small 1/2" dot or stripe painted on the end-doesn't matter what colour.for every centimeter that you can see on the ruler before the dot dissapears will tell you how much your culture is growing.1 cm=27.6 2cm=19.2 3cm=13.3 4cm=8.8 5cm=5.2 6cm=2.9-these are millions of cells for nanochloropus.you can measure this daily and record your results,but wash off the ruler or better yet get two rulers and never mix your cultures.
i get my cultures from florida aqua farms and grow them in aquamedic reactors using led lights

[ScienceTeen]

Glad ScienceTeen asked. I'll pass this on, and she can get back to you with questions. She had a different idea of a control group, but this sounds like another good suggestion. She'll give you, consultant-on-tap extraordinaire, full credit!
ScienceMum

[BlueWorldAquatic]

Way too much information.

just use 2L coke bottles and G2 Fertilizer.

Water at 1.017, slow bubbles (one every 1/2 second, not to damage the cells), and lotsa light (Flouresent prefered)

Ken - BWA

[ponokareefer]

Originally Posted by monocus

i get my cultures from florida aqua farms and grow them in aquamedic reactors using led lights

I just got my "kit" from florida aqua farms after failing to grow phyto from a store boughten brand. The kit includes a 186 page manual that has a tone of good information. I would recommend going with them seeing how thorough this kit is.

[monocus]

store bought are 99% dead cultures.they have to be shaken at least twice a day so the cultures don't smother themselves.they are also a mix of various cultures so you wouldn't get a pure strain

[BlueWorldAquatic]

I beg to differ.

We actually propogate our own live cultures, that are also avaliable in bulk.

100% live.

[monocus]

sorry-i was referring to phyto that is packaged and sent to stores,not phyto that is cultured on the premises.

[Dr_Hicks]

Originally Posted by monocus

sorry-i was referring to phyto that is packaged and sent to stores,not phyto that is cultured on the premises.

Quote from http://wiki.reefnutrition.com/w/page...20and%20Rumors

Not all Phyto shipped to retail locations is dead.

Myth Phyto-Feast Live is not alive
Reality FALSE - Phyto-Feast Live DOES contain live microalgae. The CCMP has tested it and both our biggest competitor and a Federal Judge have accepted evidence affirming that it contains live microalgae. To see a copy of the CCMP report click here.

[monocus]

glad i could help.packaged phyto might contain live algae at the start-but after a few days it would smother itself unless shaken daily.