nannochloropsis phytoplankton culture (questions)

[phyto4life]

I posted early. tomorrow at 3pm I will have the petri dish with water under light for 24 hour's

from here you say to add it straight to a salinity of around 1.017 ppm with fertilizer and bubble the heck out of it in which my pump can do because it is set to bubble a 1 gallon

my temp is probably around 70-75F

my question is how much fertilizer and what amount of pre mixed saltwater/ liter/gallon etc to use right away?

the bottle from f.a.f liquid says 1/4 tsp for 1 G @ f/2 level and double that for full strength

should I go full strength fertilizer right away?

and the petri dish says 1 liter for the entire dish to inoculate should I go more or the recommended amount at first right away?

ultimately I'll be doing 1G cultures x 8

I have a second dish so i'm open to what ever suggestions