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#1
11-05-2011, 06:28 AM
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Live Phytoplankton saltwater culture / HELP
I have a science experiment underway and have tried unsuccessfully this week to grow live phytoplankton ("Phyto Feast" from Reef Nutrition) in saltwater without success.
I don't have another week to try again, and would like to find an already-cultured sample this weekend (November 4-6) to keep going with experiment. Please call (604) 220-5032 if you can point me in the right direction to purchase, or best, for free. Nise, the Science Teen in Kitsilano, Vancouver ps happy to share my experiment idea with you! 
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#3
11-06-2011, 03:05 AM
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Nanochloropus, tetra, iso and rotofers?
Monocus, those terms are new to me, but I get what you're saying about putting the carrot back in the soil. That's about as much as I got by trying to culture my own phyto. Thanks for your offer!
Nise 
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#5
11-08-2011, 10:12 PM
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Phytoplankton
So, it's been quite a journey: bottles found, ends cut open, salt water mixed, light on, phytoplankton fed and (hopefully)
growing. It would have been a non-starter without you. Will let you know the results. Thanks for all your help! Nise the ScienceTeen
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#6
11-08-2011, 11:26 PM
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CO2 into tank?
Help again, monocus!
 We are wondering about getting the CO2 into the tank. We have the CO2 mix from a website (how does the mix below look to you?), and we'd mix it outside of the bottle. Would we use the RO water, or the salt water we've already made? We don't have the filter that's mentioned, and do we need to have one? I don't think we'd put the CO2 straight into the bottle that already has the phyto in it, but how else would we do it? Thanks. Nise. "CO2 Generator http://www.tropicalfishkeeping.com/d...generator-611/ For any of you who are interested in building your own CO2 generator, here is how it is done. Very, very easy and super cheap! You will need: 1x 2liter Soda Bottle, emptied 1cup Sugar 1tsp Yeast 1tsp Baking Powder Fresh Warm Water (not from the aquarium) CO2 Proof Tubing Small amount of silicone sealant 1) Drink the soda, preferably in one sitting. 2) Drill a hole in the soda cap large enough so the tubing will fit snuggly. 3) Push the tubing through the soda cap so it sticks out maybe 1/2 inch through the bottom of the cap. 4) Seal around the tube on the top and bottom of the soda cap, allow it to dry and make sure that it is air tight! Once the silicone is dry and it is air tight, it's time to create some CO2! 1) Add the sugar to the bottle (1 cup). 2) Add the yeast and baking powder (1 tsp each) on top of the sugar. 3) Add warm water (1 liter) to the bottle, leave the top 4-5 inches empty as this room is needed to produce the CO2. 4) Cap the bottle and put the other end of the tubing into the intake of your canister filter (in the tank). The CO2 should start being produced in a day or so, you may notice the occasional bubble being released into the intake of your canister filter. The CO2 will get all mixed up inside the filter and will dissolve into the water. If you were to just place the tube in the tank and let it bubble, the CO2 would leave the tube and float right to the top of the aquarium and would be pointless. As far as the measurements go, you can figure out what works best for you. The sugar is like food for the CO2 generation so the more sugar you use, the longer the mixture will last. The yeast is what actually reacts and creates the CO2 (along with the water and sugar of course). And the baking powder is used to help stabilize the CO2 production. The amounts stated above should last about 2 weeks. One 2 liter should be good to supply CO2 to a tank up to about 50 gallons. For larger tanks, just build more CO2 generators! By the way... You can also just plug the tubing into an airstone and let it mix with the water that way (but the canister filter method will probably work better). The CO2 may change the PH of your water."
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