Quote:
Originally Posted by Aquattro
Alternatively, lots of algae will contribute CO2 at night, which is when I lost my fish.
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Yes, that's why for this test, one could turn off the lights to increase the depletion of O2 in the tank. See Eric's clownfish tank test where his DO dropped to 16% at night. And overflows are like waterfalls I guess so that is probably oxygenating the water. If that water is coming back into the display at large volumes, then the display tank is oxygenated no matter whether lights are on or off.