I mix RODI then run it through UV sterilizer 100 micron over each vat for contaminant.
Grow Nannochloripsis to about roughly 0.2 billioncell/ml (cell size 2 µm )
Grow Tetraselmis to about 0.12 cell billion/ml (cell size 9-12 µm)
Grow rotifer to about 0.01 billion/ml (2-6 mm range)
After reaching the growth above I mix all in a container to feed daily into my system.So far the bottles I been producing will stay live for about 2 mths unless I have them all as cryo-preserved in witch case last longer refrigerated and up to 6 mths frozen.
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