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-   -   Refractometer calibration solution (http://www.canreef.com/vbulletin/showthread.php?t=124120)

hurley 08-22-2017 05:06 PM

Refractometer calibration solution
 
Does anyone know where you can pick this stuff up in Calgary?

Skimmer Juice 08-22-2017 07:18 PM

Wai's Normally carry's the calibration fluid I got a bottle there not to long ago .

Zoaelite 08-23-2017 04:14 AM

Am I missing something here? I've always calibrated to zero with RO water.

spit.fire 08-23-2017 04:31 AM

Quote:

Originally Posted by Zoaelite (Post 1017451)
Am I missing something here? I've always calibrated to zero with RO water.

same

Zoaelite 08-23-2017 05:03 AM

Okayyyy, so after a little searching apparently zeroing with RO can miscalibrate your reading. Not sure by how much considering I've never had a problem.

That being said I will do the loop, find some of this solution and get back to you guys as to how far off the reading is from zero.

Skimmer Juice 08-23-2017 05:35 AM

Quote:

Originally Posted by Zoaelite (Post 1017453)
Okayyyy, so after a little searching apparently zeroing with RO can miscalibrate your reading. Not sure by how much considering I've never had a problem.

That being said I will do the loop, find some of this solution and get back to you guys as to how far off the reading is from zero.

that is what I always did , until I was reading up on it . Ended up grabbing some from wais and it was out a fair bit , but like you never noticed any issues prior to using the fluid . And have also not had any issues since switching the calibration fluid lol

crimper 08-23-2017 06:06 AM

I always calibrate my refractometer with Distilled Water until I almost killed all my SPS as I can't figure what's wrong with my tank as all parameters seems to be right. After some googling I came across this blog:

http://blog.captive-aquatics.com/cap...sing-rodi.html

So I decided to buy a Pinpoint refractometer calibration fluid. I was shocked more than my corals about what I found out. I was running my tank salinity @ 1.030 for the longest time. :redface:

I then check other forums regarding this and there are lots of threads with this discussion.

crimper 08-23-2017 06:09 AM

A thread started by Frogger a few moons ago...

http://www.canreef.com/vbulletin/sho...ibration+fluid

Myka 08-23-2017 12:23 PM

The reason you should use calibration fluid is because the refractometers on the market for marine aquariums are almost exclusively brine refractometers, not seawater refractometers. Brine refractometers don't "see" all the salts in seawater, they only "see" a couple salts. As long as a brine refractometer is calibrated at whichever salinity you want to use, it will be accurate at that salinity only. If you're testing, say, hyposalinity with a brine refractometer calibrated to 35 ppt (1.0264 sg), it won't be accurate at 11 ppt (1.009 sg) for hypo just like it isn't accurate at 0 ppt for RO/DI or distilled water.

Most of the confusion lies in the instructions for brine refractometers which say to use distilled water because if you're testing brine with a brine refractometer then distilled water is a perfectly fine calibration solution to use. Most marine hobby brine refractometers are rebranded, such as Vertex, and they don't bother to put proper instructions for seawater calibration in the packages.

D-D and Red Sea both sell true seawater refractometers. The best ones on the market are made by VeeGee and cost about $160. If you have a true seawater refractometer then distilled wTer can be used to calibrate. You can also calibrate with seawater solution and achieve a dual point calibration which allows you to double check the calibration solution.

Calibration solution is a whole other can of worms...I've noticed they vary by as much as 5 ppt from one brand to the next. I started making my own (Randy Holmes Farley published a recipe, Google it he also explains why brine refractometers read off when calibrated with distilled water) and bought dropper bottles off Amazon (like ones used in test kits or for eye drops) to store it in so that there is no contamination or evaporation. I've found the Two Little Fishies solution to be the most accurate, however it's in a large open top bottle that's prone to evaporation/contamination very quickly even when the lid seems closed well.

kien 08-23-2017 03:21 PM

*suddenly all LFS see a huge spike in people coming in to purchase calibration fluid*

:lol:


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