why is my phytoplankton not dark like others

[donkey77]

this is day 8 and i'am about to split the culture and when i put it into bottles it was not that green, did i not let it go long enough, just seems to light of green i will go to day 10 but any input would be nice
http://i564.photobucket.com/albums/ss86/donkey1977/IMG_2046.jpg (http://s564.photobucket.com/user/donkey1977/media/IMG_2046.jpg.html)

[Cal_stir]

are you using fertilizer?
are you culturing rotifers close by, if contaminated with rotifers they will eat as fast as it grows.
light for 16 hrs a day?

[donkey77]

no rotifers giving f2
light 16hrs

[Cal_stir]

what kind of light?

[donkey77]

i think its a led it's the kit from mamroo200

[Cal_stir]

I think they are flourescent, which should work fine.
What about temp., and you have an air line running like a slow boil.
How much f2?

[donkey77]

temp is about 72 74
airstone to a slow boil
this is my first batch
3ml f2 for about 3 gallons

[Cal_stir]

I would split it and put a little more f2 this time, an extra ml, are you using tap water or RO?
I used filtered water ( just after the carbon block of my RODI unit), salinity 1.019, don't use RODI

[donkey77]

iam using RO water so i should be using tap water, what about all the crap in tap water

[donkey77]

do you put yours in the fridge

[The Guy]

temp is about 72 74
airstone to a slow boil
this is my first batch
3ml f2 for about 3 gallons
I was told not to use an air stone only a hard tube open ended at the bottom of the bottle, also I use a 6500 k compact bulb.
I'm new to this so we'll see how it goes. :neutral:

[Cal_stir]

RO water is too pure, I used carbon filtered water and yes no air stone just a piece of rigid tubing.
the light should be ok if it came in mamroo's kit, he grows nice green phyto.
6500k cfl works good.
what phyto you growing?

[monocus]

most likely it has to brew for a longer period of time.what strain of phyto are you using?i use r/o water at 1.025 salinity run through an uv sterilizer.for a gallon of phyto i use about 3 ml of micro algae grow.lighting i made my own led strips using 1 watt leds(25 per strip)various cultures like different lights(nanochloropus-white/red,tetraselmus-red,isocrysis-blue-etc)i'm using aquamedic reactors for my cultures and rotifers